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目的目的了解吉林省活禽市场外环境禽流感病毒分布状况。方法于2014年3月1日—10日采集吉林省活禽市场外环境,共抽取370个采样点,每个点抽取50家活禽摊位采集外环境鸡笼涂抹、鸡粪和污水标本,采用实时荧光定量PCR法检测禽流感病毒FluA H5、H9和H7核酸。结果 370个采样点中58个(15.68%)检出阳性标本,各地区标本核酸阳性率有明显差异(P<0.05);共采集活禽市场外环境标本1 110份,分别为污水359份,粪便381份,笼具擦拭物370份,其中阳性94份,H9阳性60份,H5阳性30份,H5/H9混合阳性4份,未检测出H7阳性,不同类型标本的阳性检出率无统计学差异。结论吉林省活禽市场环境存在禽流感病毒的污染,主要型别为H9和H5亚型,未发现H7亚型。
Objective To understand the distribution of avian influenza virus in live environment in live poultry market in Jilin Province. Methods The external environment of live poultry market in Jilin Province was collected from March 1 to March 10, 2014. A total of 370 sampling sites were sampled and 50 live poultry stalls were sampled at each site to collect external environment chicken cages, chicken manure and sewage samples. Real-time Fluorescent Quantitative PCR for the FluA H5, H9 and H7 Avian Flu Virus. Results Positive samples were detected in 58 out of 370 sampling points (15.68%), and the positive rate of nucleic acid in each area was significantly different (P <0.05). A total of 1101 environmental samples were collected from live poultry market, including 359 sewage samples, 381 feces and cage with 370 swabs, of which 94 were positive, 60 were positive for H9, 30 were H5 positive and 4 were H5 / H9 mixed positive, no positive H7 was detected. The positive detection rate of different types of specimens was not statistically significant Differences Conclusions The live poultry market environment in Jilin Province is contaminated with avian influenza virus. The main types are H9 and H5 subtypes, and no H7 subtype is found.