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据《中华妇产科杂志》2001年10月36卷第10期报道 河北医科大学第四医院妇产科吴小华等,为探讨基质金属蛋白酶-2(MMP-2)的基因和蛋白在卵巢上皮性肿瘤中的表达以及与临床病理特征和预后的关系,采用半定量逆转录聚合酶链反应(RT-PCR)、SP免疫组织化学方法,对41例卵巢上皮性癌及26例卵巢良性肿瘤MMP-2基因及蛋白的表达,分别进行了定量和定位分析。应用蛋白印迹法定性检测MMP-2酶原及活性MMP-2在卵巢上皮性肿瘤中的表达。
According to the “Chinese Journal of Obstetrics and Gynecology,” October 2001 Volume 36, No. 10 report of the Fourth Hospital of Hebei Medical University Wu Xiaohua and so on, to explore the matrix metalloproteinase-2 (MMP-2) gene and protein in ovarian epithelial Tumor and its relationship with clinicopathological characteristics and prognosis. The mRNA expression of MMP-2 in 41 cases of ovarian epithelial carcinoma and 26 cases of benign ovarian tumor was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and SP immunohistochemistry. 2 gene and protein expression, respectively, quantitative and positional analysis. Western blotting was used to detect the expression of MMP-2 and MMP-2 in epithelial ovarian tumors.