用ＲＴＰＣＲ方法对２５０例孕产妇宫颈分泌物、粪便、及新生儿出生后３天内粪便，进行轮状病毒检测。结果孕产妇ＨＲＶ肠道感染率为２６％，宫颈分泌物ＨＲＶ阳性为７２％。ＨＲＶ感染的孕妇是新生儿ＨＲＶ感染的主要传染源；尤其是宫颈分泌物ＨＲＶ阳性孕妇其新生儿有５０％受染；同时出生３天内的新生儿ＨＲＶ感染随天龄增加。结论：ＨＲＶ感染的孕妇分娩过程是其新生儿ＨＲＶ感染的重要感染源，与新生儿接触的物品及工作人员的手等是院内感染的重要传播途径。 The RT-PCR method was used to detect rotavirus in 250 cases of pregnant women cervical secretions, faeces, and within 3 days after birth of newborns. Results The rate of intestinal infection of pregnant women HRV was 26%, HRV positive of cervical secretions was 72%. HRV infection of pregnant women is the main source of infection of neonatal HRV infection; especially cervical secretions of HRV-positive pregnant women, 50% of their newborns were infected; also born within 3 days of neonatal HRV infection increased with age. Conclusion: Pregnant women with HRV infection during labor are the important source of HRV infection in their newborns. The items that are in contact with neonates and the hands of staff are important routes of nosocomial infection.