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目的:建立高效液相色谱-质谱联用法测定人血浆中兰索拉唑的浓度。方法:血浆样品经处理后,采用Waters AtlantisC_(18)色谱柱(100 mm×2.1 mm,3μm),流动相为甲醇-0.01%甲酸溶液(70:30),以电喷雾电离源(ESI)正离子检测,奥美拉唑为内标。结果:兰索拉唑血浆质量浓度的线性范围为5.0～1 500.0 ng·ml~(-1)(r=0.999 8),,提取回收率为78.2%～88.9%(n=5),方法回收率为90.3%～101.9%(n=5),日内和日间RSD均小于10%。结论:本法专属性强,样品处理方便,灵敏度高,适用于兰索拉唑药动学研究。
Objective: To establish a method for the determination of lansoprazole in human plasma by high performance liquid chromatography-mass spectrometry. Methods: The plasma samples were separated on a Waters Atlantis C 18 column (100 mm × 2.1 mm, 3 μm). The mobile phase consisted of methanol-0.01% formic acid solution (70:30) and electrospray ionization source Ion detection, omeprazole as internal standard. Results: The linear range of plasma concentration of lansoprazole was 5.0-1 500.0 ng · ml -1 (r = 0.999 8), and the extraction recovery was 78.2% -88.9% (n = 5) Rates ranged from 90.3% to 101.9% (n = 5) with daily and intraday RSDs less than 10%. Conclusion: This method is highly specific, convenient sample processing, high sensitivity, suitable for lansoprazole pharmacokinetic studies.