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目的对河北省血液中心2011年-2013年血源梅毒认定为不合格的样本进行确证并反馈。方法收集河北省血液中心2011年1月-2013年12月梅毒螺旋体抗体ELISA检测判定为不合格的标本;以梅毒螺旋体明胶凝集试验(TPPA)对其进行确证试验;专职人员通过966016献血服务平台或面谈反馈结果。结果 ELISA判定为不合格的标本为1 446份。经TPPA法确证,778例为阳性,阳性率为53.80%;当S/CO>5,阳性符合率为97.79%;当S/CO为1~5,阳性符合率为66.33%。反馈服务满意率为98.72%。结论以TPPA确证梅毒ELISA的筛查结果,在得到准确检测结果,提高血站服务质量的同时,可为相关医学发展提供数据支持。及时反馈则可降低对献血者的身心伤害;促进献血者归队,减少血源流失。
Objective To confirm and give feedback on the samples of blood syphilis identified as unqualified from 2011 to 2013 in Hebei Blood Center. Methods Blood samples collected from Hebei Provincial Blood Center from January 2011 to December 2013 were collected and tested by ELISA for detection of Treponema pallidum antibody. The test was confirmed by TPPA. The full-time staff members passed 966016 blood donation service platform or Interview feedback results. Results 1 446 samples were judged as unqualified by ELISA. According to TPPA method, 778 cases were positive and the positive rate was 53.80%. When S / CO> 5, the positive coincidence rate was 97.79%. The positive coincidence rate was 66.33% when S / CO was 1-5. Feedback service satisfaction rate was 98.72%. Conclusions TPPA confirmation of syphilis ELISA screening results, accurate test results, improve blood service quality, while providing relevant data for the development of medical support. Timely feedback can reduce the physical and psychological damage to blood donors; promote blood donors rejoin, reduce blood loss.