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目的 比较评价儿童肾小球疾病血浆、血清、尿液不同标本的转化型生长因子 β1(TGF β1)水平。方法 用ELISA方法测定 30例轻微病变、30例系膜增生性肾小球肾炎、2 4例IgA肾病、2 4例局灶节段性肾小球硬化患儿和 30例健康对照组儿童血浆、血清、尿液中的TGF β1水平。结果 尿液标本的TGF β1水平对各组的诊断有显著意义 (P <0 0 5 ) ,而血浆、血清标本没有诊断意义 (P >0 0 5 )。血浆测定TGF β1灵敏度较血清好。血浆、尿液指标有较好的相关性 (r=0 85 ,P <0 0 5 ) ;血清与尿液仅有一定的相关性 (r=0 5 3,P <0 0 5 ) ;血浆与血清无显著相关性 (r =0 4 9,P >0 0 5 )。结论 尿液为检测肾炎患儿TGF β1水平的较好指标 ,血浆次之 ,血清最差。
Objective To compare the levels of transforming growth factor β1 (TGFβ1) in different samples of plasma, serum and urine in children with glomerular disease. Methods Thirty patients with mild disease, 30 patients with mesangial proliferative glomerulonephritis, 24 patients with IgA nephropathy, 24 children with focal segmental glomerulosclerosis and 30 healthy controls were measured by ELISA. Serum, urine TGF β1 levels. Results The level of TGFβ1 in urine samples was significantly higher than that in other groups (P <0.05). However, serum and serum samples did not have diagnostic significance (P> 0.05). Determination of plasma TGF β1 sensitivity than serum. Plasma and urine indexes had a good correlation (r = 0 85, P <0 05). There was only a certain correlation between serum and urine (r = 0 53, P 0 05) Serum no significant correlation (r = 0 49, P> 0 0 5). Conclusion Urine is a good indicator of TGFβ1 level in children with nephritis, followed by plasma and serum in the worst.