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背景:微量元素与人体健康的关系日益受到重视,许多微量元素是机体必不可少的。微量元素的缺乏与心脑血管疾病的发生有着不容忽视的密切关系,而中药的药效则决定于它所含微量元素的种类和含量。目的:测定10种治疗脑血管疾病的中成药中钙(Ca)、镁(Mg)、铜(Cu)、铬(Cr)、锌(Zn)和铁(Fe)6种金属元素的含量。单位:吉林医药学院医学实验中心。材料:实验于2003-03/2003-05在吉林化工学院进行。选择脑塞通、强力天麻杜仲丸、抗栓丸、通脉宁心冲剂、溶栓胶囊、步长脑心通、大活络丹、中风回春丸、消栓通络胶囊、华佗再造10种治疗心脑血管疾病的中成药,均为市售。浓HNO3,优级纯,北京化工厂生产;HClO4,优级纯,天津市东方化工厂生产。方法:将样品丸剂取10丸,片剂取10片,分别粉碎混匀。每一样品平行取2份,每份1.00g,分别置于10个50mL锥形瓶中,加入浓HNO310mL封口过夜。次日在锥形瓶中分别加入浓HNO310mL,HClO45mL,于电热板上缓慢加热,至剩余2mL左右时再分别加入浓HNO320mL,HClO45mL,继续消化至溶液呈无色透明近干为止,冷却后分别将药品转移至25mL容量瓶中,用3%HNO3定容。采用原子吸收分光光度法分别测定10种中成药中Ca、Mg、Cu、Cr、Zn、Fe6种金属元素的含量。主要观察指标:10种中成药中Ca、Mg、Cu、Cr、Zn、Fe6种金属元素的含量。结果:10种药物中金属元素含量:溶栓胶囊中Mg含量较多,为(89.2016±0.8554)mg/g;大活络丹中Ca含量最多,为(14.3125±0.5070)mg/g;抗栓丸中Zn、Fe含量丰富,分别为(60.4875±1.6398)mg/g和(980.0812±15.3959)mg/g;而消栓通络胶囊中含Cr较多,为(9.8812±0.7344)mg/g。结论:10种药物中的金属元素含量都比较丰富,但存在一定差异。显示结果为探讨中成药金属元素含量与治疗脑血管疾病疗效的关系提供了一定依据。
Background: The relationship between trace elements and human health is increasingly valued, and many trace elements are essential to the body. The lack of trace elements has a close relationship with the occurrence of cardiovascular and cerebrovascular diseases. The efficacy of traditional Chinese medicine depends on the type and content of the trace elements it contains. OBJECTIVE: To determine the contents of six metal elements Ca, Mg, Cu, Cr, Zn and Fe in 10 Chinese medicines for the treatment of cerebrovascular diseases. Unit: Jilin Medical College Medical Experimental Center. MATERIALS: The experiment was conducted at the Jilin Institute of Chemical Technology from March 2003 to May 2003. Selecting Naoxicai Tong, Qiang Li Tian Ma Du Zhong Wan, Kang Shu Wan, Tong Mai Ning Xin Granules, thrombolytic capsules, Buchang Naoxintong, Daowoluodan, Zhongfeng Huichun Pills, Xiaoshuan Tongluo Capsules, and Huatan Rebuilding 10 treatments Chinese medicines for cardiovascular and cerebrovascular diseases are all commercially available. Concentrated HNO3, superior grade pure, produced by Beijing chemical plant; HClO4, superior grade pure, produced by Tianjin Dongfang Chemical Factory. Method: Take 10 pills of sample pills, 10 tablets, and crush and mix. Two samples of each sample were taken in parallel and each 1.00 g was placed in 10 50 mL Erlenmeyer flasks and blocked with HNO 310 mL overnight. The next day in a conical flask were respectively concentrated HNO310mL, HClO45mL, slowly heated on a hot plate, to the remaining 2mL or so, then add concentrated HNO320mL, HClO45mL, continue to digest until the solution was colorless, transparent and nearly dry, respectively, after cooling Drugs were transferred to a 25 mL volumetric flask and brought to volume with 3% HNO3. The contents of six metal elements Ca, Mg, Cu, Cr, Zn, and Fe in 10 Chinese patent medicines were determined by atomic absorption spectrophotometry. MAIN OUTCOME MEASURES: The contents of six metal elements Ca, Mg, Cu, Cr, Zn, and Fe in 10 proprietary Chinese medicines. Results: The content of metal elements in 10 drugs: Mg content in thrombolytic capsules was (89.2016±0.8554) mg/g; the content of Ca in Daluoluodan was the highest (14.3125±0.5070) mg/g; The contents of Zn and Fe in the samples were (60.4875±1.6398) mg/g and (980.0812±15.3959) mg/g, respectively, while those in Xiaoshuan Tongluo capsules were more (9.8812±0.7344) mg/g. Conclusion: The contents of metal elements in 10 kinds of drugs are relatively abundant, but there are some differences. The results provide a certain basis for exploring the relationship between the content of metal elements in Chinese medicine and the treatment of cerebrovascular disease.