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用磷酸盐缓冲液在 80℃时从灵芝菌丝体中提取出多糖 -蛋白质缀合物 LM-A.成分分析表明 ,GLM-A主要由葡萄糖组成 ,蛋白质含量为 1 3 % . GLM-A在 1∶ 1水稀释的饱和镉乙二胺溶液中 3 0℃时的特性粘数 [η]和均方根旋转半径〈S2 〉1 /2 的分子量依赖关系分别为 [η]=5.1× 1 0 - 2 M0 .6 0w ( cm3· g- 1 )和〈S2 〉1 /2 =3 .9×1 0 - 2 M0 .5 0w ( nm) .按照 Yamakawa-Fujii-Yoshizaki蠕虫状圆筒模型的粘度理论和 Bohdanecky表达式 ,求得GLM-A的分子参数为 :单位围长摩尔质量 ML=( 53 0± 1 0 ) nm- 1 ,持久长度 q=( 2 .8± 0 .2 ) nm.链直径 d=0 .75nm.实验结果表明 ,GLM-A在该溶液中为无规线团构象
The polysaccharide-protein conjugate LM-A was extracted from the mycelium of G. lucidum with phosphate buffer at 80 ° C. Composition analysis showed that GLM-A was composed mainly of glucose with a protein content of 13%. GLM-A The molecular weight dependence of the intrinsic viscosity [η] at 30 ° C and the root mean square radius of gyration 1/2 on a 1: 1 water-saturated saturated cadmium ethylenediamine solution is [η] = 5.1 × 10 - 2 M0.60w (cm3 · g-1) and 1/2 = 3.9 × 10-2 M0.5w (nm) According to the viscosity of the worm-shaped cylinder model of Yamakawa-Fujii-Yoshizaki Theory and Bohdanecky expression, the molecular parameters of GLM-A are obtained as follows: the unit circumference length of the molar mass ML = (53 0 ± 1 0) nm-1, the persistence length q = (2 .8 ± 0 2) nm. Diameter d = 0.7575 nm The experimental results show that GLM-A is a random coil conformation in this solution