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目的:探讨外源性骨髓间充质干细胞(MSCs)移植对缺血再灌注损伤(I/R)后肾小管上皮细胞增殖和凋亡的影响。方法:将雄性SD大鼠MSCs用DAPI标记后注入受体雌性SD大鼠体内。30只受体大鼠随机分为3组:假手术对照组(C组)、MSCs+I/R组(M组)、DMEM-F12+I/R组(D组),每组10只。7d后观察肾功能,肾脏病理改变,采用原位末端标记法检测细胞凋亡指数,免疫组化法检测增殖细胞核抗原(PCNA)的表达,并观察DAPI标记的MSCs在受体大鼠肾脏的分布情况。结果:I/R后第7天,M组在肾功能、肾脏病理改变上,均明显好于D组;肾组织内PCNA+细胞数和凋亡指数均低于D组。I/R后7d内未发现MSCs定位于肾组织中。结论:外源性MSCs可以促进I/R损伤后肾小管上皮细胞的增殖和减少细胞凋亡,从而有利于肾小管损伤的早期恢复。
Objective: To investigate the effects of exogenous bone marrow mesenchymal stem cells (MSCs) transplantation on the proliferation and apoptosis of renal tubular epithelial cells after ischemia / reperfusion injury (I / R). Methods: Male SD rats were labeled with DAPI and injected into female SD rats. Thirty rats were randomly divided into three groups: sham operation control group (C group), MSCs + I / R group (M group) and DMEM-F12 + I / R group (D group) After 7 days, the renal function and renal pathological changes were observed. The apoptotic index was detected by in situ end labeling. The expression of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry. The distribution of DAPI-labeled MSCs in the kidney of recipient rats Happening. Results: On day 7 after I / R, M group was significantly better than D group in renal function and renal pathological changes. The number of PCNA + cells and apoptotic index in renal tissue were lower than those in D group. MSCs were not found localized in the kidney within 7 days after I / R. Conclusion: Exogenous MSCs can promote the proliferation of renal tubular epithelial cells and reduce apoptosis after I / R injury, which is beneficial to the early recovery of renal tubular injury.