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探讨新生儿不同病原感染性肺炎γ-干扰素(IFN-γ)和补体急性相蛋白(C_3、C_4和B因子)的变化及其意义。采用免疫酶法(ELISA)、速率散射比浊法检测肺炎患儿血清IFN-γ、C_3、C_4、B因子(Bf)含量。以CRP≥20mg/L作为诊断细菌性感染的界限值,结合其它临床资料,将肺炎患儿分为4组进行结果分析。结果:(1)肺炎病因学检测:肺炎组111份血清中,8型常见病毒及支原体特异性IgM阳性40份(36.04％);对照组30份血清均阴性。病毒及支原体肺炎23例(20.7％),细菌性肺炎45例(40.5％),病毒及支原体合并细菌性肺炎17例(15.3％),其它肺炎26例(23.4%)。(2)IFN-γ和补体急性相蛋白含量检测:IFN-γ、C_3、C_4和Bf含量在肺炎各组与对照组之间差异无统计学意义(P>O.05)。结论:病毒及支原体、细菌、病毒及支原体合并细菌感染和其它感染为本地区新生儿肺炎的重要病因。体液中发挥非特异性免疫功能的IFN-γ、补体C_3、C_4和Bf含量不足,是新生儿容易发生感染性肺炎的原因之一。
To investigate the changes and significance of interferon-γ (IFN-γ) and acute phase protein (C_3, C_4 and B factor) in neonates with different pathogenic infectious pneumonia. The contents of IFN-γ, C_3, C_4, B factor (Bf) in children with pneumonia were detected by enzyme-linked immunosorbent assay (ELISA) and rate nephelometry. To CRP ≥ 20mg / L as a diagnostic threshold for bacterial infections, combined with other clinical data, the children with pneumonia are divided into four groups for analysis of the results. Results: (1) Etiological analysis of pneumonia: Among the 111 serums in pneumonia group, 8 were common virus and 40 were specific mycoplasma-specific IgM (36.04%), while 30 in the control group were all negative. There were 23 cases (20.7%) of virus and mycoplasma pneumonia, 45 cases (40.5%) of bacterial pneumonia, 17 cases (15.3%) of virus and mycoplasma with bacterial pneumonia and 26 cases (23.4%) of other pneumonia. (2) The content of IFN-γ and acute phase protein of complement: The levels of IFN-γ, C_3, C_4 and Bf in pneumonia were not significantly different between the groups and the control group (P> 0.05). Conclusion: The combined infection of the virus, mycoplasma, bacteria, viruses and mycoplasma and other infections are the important causes of neonatal pneumonia in this area. The non-specific immune function of body fluid IFN-γ, complement C_3, C_4 and Bf content is insufficient, is one of the reasons neonatal pneumonia prone.