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为探讨贝类杂交优势 ,利用HOECHST3 3 2 5 8对已固定样品进行染色的方法 ,连续观察了栉孔扇贝♀×华贵栉孔扇贝♂受精的细胞生物学过程 ,初步证明了这 2种远缘扇贝种类之间进行杂交的可行性。结果表明 ,栉孔扇贝的成熟未受精卵子处于第一次减数分裂中前期 ,部分来自华贵栉孔扇贝的异源精子可顺利进入卵子并激发后者完成 2次减数分裂 ,排出第一及第二极体。成熟的雌雄原核形成后完成融合过程 ,形成合子核 ,受精过程结束。大部分杂交受精卵及杂种早期胚胎可以正常发育 ,但其发育进程较种内近交对照组有明显的差别 ,具体表现在延缓性和不同步性 2个方面。
In order to explore the superiority of shellfish hybridization, the cell biological process of ♂ fertilization of scallop Chlamys farreri ♀ × Scallop 扇 scallop was observed continuously by using the method of HOECHST3 3285 to stain the fixed samples. Feasibility of hybridization between scallop species. The results showed that the mature and unfertilized eggs of scallop scallop were in the first stage of meiosis. Some of the sperm from the scallop (Scallop and Scallop) could successfully enter the egg and stimulate the latter to complete 2 meiosis, The second polar body. Mature male and female pronucleus after the formation of the fusion process to form a zygote nucleus, fertilization process is over. Most of the cross fertilized eggs and hybrid early embryos can be normal development, but its development process than the inbred control group were significantly different, specifically in the delay and asynchronous 2 aspects.