论文部分内容阅读
目的:通过检测类风湿性关节炎(RA)患者血清粒细胞-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)水平,探讨其用于RA诊断与预后判断的临床意义。方法:选取我院2013年6月~2016年12月收治的126例RA患者为RA组,并根据病情严重程度分为活动期RA组(69例)和缓解期RA组(57例)两个亚组;另选取同期于我院体检中心接受体检的55例健康体检者为对照组。采用酶联免疫吸附法(ELISA)检测所有对象的血清GM-CSF、IL-6、TNF-α水平。记录比较各组血清细胞因子水平,并分析RA组患者各细胞因子水平及DAS28间的相关性。结果:活动期RA组的血清GM-CSF、IL-6、TNF-α水平均显著高于缓解期RA组和对照组(P<0.01),缓解期RA组的血清GM-CSF、IL-6、TNF-α水平亦均显著高于对照组(P<0.01)。血清GM-CSF、IL-6及TNF-α水平三者间均呈显著正相关(P<0.01);DAS28与血清GM-CSF、IL-6、TNF-α水平亦均呈显著正相关(r=0.473、0.584、0.675,P均<0.01)。结论:类风湿性关节炎患者的血清GM-CSF、IL-6、TNF-α水平均显著上调,且与类风湿性关节炎的活动性呈显著正相关,通过检测此类细胞因子对类风湿性关节炎的早期诊断、病情评估及判断预后均具有重要临床意义。
Objective: To detect the levels of serum GM-CSF, IL-6 and tumor necrosis factor-α (TNF-α) in patients with rheumatoid arthritis (RA) Level, to explore its clinical significance for diagnosis and prognosis of RA. Methods: A total of 126 RA patients admitted to our hospital from June 2013 to December 2016 were selected as RA patients and divided into active RA group (69 cases) and remission RA group (57 cases) according to the severity of the disease Another group of 55 healthy people undergoing physical examination during the same period in our hospital physical examination center were selected as the control group. Serum levels of GM-CSF, IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA). The levels of serum cytokines in each group were compared and recorded. The levels of cytokines and the association between DAS28 in RA patients were analyzed. Results: Serum levels of GM-CSF, IL-6 and TNF-α in active RA group were significantly higher than those in remission RA group and control group (P <0.01), serum GM-CSF and IL- , TNF-α levels were also significantly higher than the control group (P <0.01). Serum levels of GM-CSF, IL-6 and TNF-α were positively correlated with each other (P <0.01). The levels of serum GM-CSF, IL-6 and TNF- = 0.473,0.584,0.675, P <0.01). CONCLUSIONS: Serum levels of GM-CSF, IL-6 and TNF-α in patients with rheumatoid arthritis are significantly up-regulated and positively correlated with the activity of rheumatoid arthritis. By examining the effect of these cytokines on rheumatoid arthritis Early diagnosis of arthritis, disease assessment and prognosis are of important clinical significance.