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目的：研究腺病毒载体介导的骨形态发生蛋白9（BMP-9）对小鼠永生化颅骨间充质祖细胞（iCAL）成骨分化的诱导作用。方法以iCAL为模型，设置绿色荧光蛋白（ GFP）对照组和BMP-9诱导组，于诱导后的3、5、7 d对早期成骨分化指标碱性磷酸酶（ ALP）活性进行定性和定量检测；于诱导后的14 d通过茜素红染色检测骨钙结节形成；荧光定量PCR法和Western印迹检测成骨细胞分化标志基因Runx2、OCN表达情况。结果 BMP-9诱导组细胞内ALP活性高于GFP对照组，差异具有统计学意义（P＜0．05）；BMP-9诱导组形成较多的钙盐结节沉积；荧光定量PCR检测结果显示诱导组中Runx2、OCN的mRNA表达量明显上调，差异具有统计学意义（P＜0．05），Western印迹检测3 d Runx2蛋白表达上调，5 d OCN蛋白表达上调。结论 BMP-9促进小鼠永生化iCAL成骨分化。“,”Objective To investigate the effect of adenovirus-bone morphogenic protein 9 ( Ad-BMP9 ) on osteogenic differentiation of immortalized calvarial mesenchymal progenitor cells ( iCALs ) .Methods iCALs were infected with adenoviral vectors encoding BMP-9 or green fluorescent protein ( GFP) and the early osteogenic differentiation was assessed by detecting alkaline phosphatase (ALP) activity after being cultured for 3, 5 and 7 days.14 days after infection, alizarin red S staining was performed to study the formation of osteogenic calcium nodules .The expression of osteogenic marker genes Runx2 and OCN was assessed by quantitative real-time ( RT )-PCR and Western blotting .Results Significant increases in ALP activity and in the expressions of Runx 2 and OCN were detected in BMP-9 treated iCALs compared with GFP-treated cells(P<0.05).Meanwhile, alizarin red S staining showed that more mineralized nodules were found in the BMP-9 induced group .Conclusion BMP-9 can promote the osteogenic differentiation of iCALs .