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目的:旨在测定不同烘制温度下党参炮制品中党参炔苷的含量,为科学评价及有效控制其质量提供可靠方法。方法:采用HPLC法、梯度洗脱及超声提取法。色谱条件为:色谱柱:Kromasil C18色谱柱(4.6 mm×250 mm,5μm);流速:1.0 m L/min;检测波长:党参炔苷267 nm,柱温:25℃;流动相:乙腈-0.05%磷酸梯度洗脱系统。结果:通过对比分析不同烘制温度下得到的炮制品,测定条件相同时,烘制党参中党参炔苷的含量高于生品;烘制温度为40℃时,蜜炙党参中的党参炔苷含量较高,烘制温度与党参中党参炔苷的含量无线性关系;蜜炙党参自然干燥品中党参炔苷含量较低。结论:不同烘制温度对党参中党参炔苷含量有一定影响;蜜炙党参最佳烘制温度为40℃。同时,采用HPLC方法测定党参中党参炔苷的含量,操作简单,结果可靠,重现性好,可作为甘肃道地药材党参及其炮制品质量控制的有效方法。
Objective: To determine the content of Codonophorin in Codonopsis products at different baking temperatures, and to provide a reliable method for scientific evaluation and effective control of its quality. Methods: HPLC, gradient elution and ultrasonic extraction were used. The chromatographic conditions were as follows: column: Kromasil C18 column (4.6 mm × 250 mm, 5 μm); flow rate: 1.0 m L / min; % Phosphoric acid gradient elution system. Results: By comparing the processed products obtained under different baking temperatures, the content of C_ (CN) ginsenoside in the dried Codonopsis pilosulae was higher than that of the raw product under the same test conditions. When the baking temperature was 40 ℃, the content of C_ High, baking temperature and Codonopsis in the concentration of Codonoposide in a non-linear relationship; Honey Sunburn Codonopsis natural dried products in lower concentrations of Codiparitin. Conclusion: The effects of different baking temperatures on the content of Codonopin in Codonopsis have some effects; the best baking temperature of Cangzhu Codonopsis is 40 ℃. At the same time, HPLC method for the determination of Codonoprazole in Codonopsis content, simple operation, the results are reliable, reproducible, and can be used as Gansu authentic Codonopsis and its processed products quality control an effective method.