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以HLA DRB基因类扩增为参照 ,通过HLA DR2组特异性扩增确定HLA DR2携带者 ,PCR/SSP作亚型分型 ,并用银染PCR/SSCP分析HLA DR2等位基因第二外显子单链构像 ,调查湖南地区汉族群体SLE(系统性红斑狼疮 )患者 5 8例和健康对照 5 9例HLA DR2等位基因分布。结果示 ,HLA DR2与SLE相关 (RR =2 .71,P <0 .0 1) ,HLA DRB1 15 0 1为疾病相关等位基因 (RR =3 .10 ,Pc <0 .0 5 ) ,该群体中检出的另外两种亚型DRB1 15 0 2 ,160 2在两组间频率分布差异无显著性。此外 ,PCR/SSCP分析表明湖南汉族HLA DR2等位基因在第二外显子内不存在其他序列变异。
Using HLA DRB gene amplification as reference, HLA DR2 carriers were identified by HLA DR2 group specific amplification and PCR / SSP was subtyped and the second exon of HLA DR2 allele was analyzed by silver staining PCR / SSCP Single-strand conformation, to investigate the distribution of HLA DR2 alleles in 58 Han patients with SLE (systemic lupus erythematosus) and 59 healthy controls in Hunan province. The results showed that HLA DR2 was associated with SLE (RR = 2.71, P <0.01) and HLA DRB1 15 0 1 was disease-associated allele (RR = 3.10, Pc <0.05) The other two subtypes, DRB1 15 0 2, 160 2, detected in the population showed no significant difference in frequency distribution between the two groups. In addition, PCR / SSCP analysis showed that there was no other sequence variation in HLA-DR2 allele of Hunan Han in the second exon.