[Objective] This study aimed to investigate the acaricidal effect of scopoletin, and provide the biochemical evidences of scopoletin influences Ca2 +-ATPase activity and gene expressions in the Carmine Spider Mite, Tetranychus cinnabarinus.[Method] The acaricidal effects of scopoletin were investigated by slip-dip method.Exposeed to different concentrations of scopoletin(0.16-2.5 mg/ml), Ca2+-ATPase activity in vivo and protein contents were investigated. For assessing the in vitro effect, Ca2+-ATPase enzyme(200 μl) prepared from normal mites were incubated with different concentrations of scopoletin reagents. [Result] Scopoletin exhibited significant inhibitory effect on Ca2+-ATPase activity both in vivo and in vitro, and resulted in increased protein contents; kinetic analysis showed that the catalytic capability of Ca2+-ATPase was significantly reduced by scopoletin. [Conclusion] Scopoletin exhibits a significant inhibitory effect on Ca2 +- ATPase, and its acaricidal effect against T. cinnabarinus might be due to the direct inhibition of Ca2+-ATPase. [Objective] This study aimed to investigate the acaricidal effect of scopoletin, and provide the biochemical evidences of scopoletin influences Ca2 + -ATPase activity and gene expressions in the Carmine Spider Mite, Tetranychus cinnabarinus. [Method] The acaricidal effects of scopoletin were investigated by Ca 2+ -ATPase activity in vivo and protein contents were investigated. For assessing the in vitro effect, Ca 2+ -ATPase enzyme (200 μl) prepared from normal mites were incubated with different concentrations of scopoletin reagents. [Result] Scopoletin Express significant inhibitory effect on Ca2 + -ATPase activity both in vivo and in vitro, and resulted in increased protein contents; kinetic analysis showed that the catalytic capability of Ca2 + -ATPase was significantly reduced by scopoletin. [Conclusion] Scopoletin exhibits a significant inhibitory effect on Ca 2+ - ATPase, and its acaricidal effect against T. cinnabarinus might be due to direct inhibition of Ca2 + -ATPase.