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应用全细胞式记录膜片钳技术,在20个从豚鼠耳蜗分离出的外毛细胞中,研究尼莫地平对豚鼠耳蜗外毛细胞钙电流的影响。结果表明,尼莫地平可部分阻断豚鼠耳蜗外毛细胞电压依赖性钙电流,其阻断作用可逆,抑制率与尼莫地平的浓度有关,其半数抑制浓度为60.14nmol/L,对电流的最大抑制率为34.16％。证实尼莫地平对外毛细胞电压依赖性通道有阻滞作用,提示其对于病理状态下持续去极化电压导致的毛细胞钙内流有较大阻滞作用而对生理状态下短暂去极化电压导致的钙内流无明显阻滞作用。认为尼莫地平在治疗梅尼埃病等内耳疾患方面有潜在价值。
The effect of nimodipine on cochlear outer hair cells calcium currents in guinea pigs was studied using whole-cell recording patch-clamp technique in 20 outer hair cells isolated from guinea pig cochlea. The results showed that nimodipine could partially block the voltage-dependent calcium current in guinea pig cochlear outer hair cells, and its blocking effect was reversible. The inhibition rate was related to the concentration of nimodipine. The half inhibitory concentration was 60.14nmol / L, The maximum inhibition rate was 34.16%. Confirmed that nimodipine has a blocking effect on the voltage-dependent channel of the hair cell, suggesting that it has a great retardation on the intracellular calcium influx caused by the sustained depolarization voltage in the pathological state and the transient depolarization voltage Lead to calcium influx no significant retardation effect. Nimodipine is considered potentially valuable in treating inner ear conditions such as Meniere’s disease.