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目的:建立裸鼠皮下人肝癌种植瘤模型。方法:BLBA/c裸鼠颈背部皮下注射人肝癌SMMC-7721细胞悬液5×106个/(0.2ml·只),观察肿瘤生长情况,10周处死。瘤组织作病理及电镜检查;取种植裸鼠周围血作甲胎蛋白(AFP)的检测,随机抽出10只未行处理的BLBA/c雄性裸鼠作对照;用免疫组化法检测肿瘤微血管密度(MVD),取正常非瘤组织作对照。结果:种植裸鼠周围血甲胎蛋白(AFP)为(528.35±12.48)ng/ml,对照组为(135.37±9.27)ng/ml,两组比较,有显著性差异(P<0.05);种植裸鼠平均每中倍视野中的微血管数为(32.45±2.38)个。对照组为(9.16±1.21)个,两组比较,有显著性差异(P<0.05)。该模型具有类似人原发性肝癌的形态学特征。结论:成功建立了人肝癌裸鼠皮下种植瘤模型。
Objective: To establish a model of human hepatocellular carcinoma in nude mice. Methods: The nude mice were injected subcutaneously with 5 × 106 cells of SMMC-7721 cells in the back of nude mice on the back of BLBA / c nude mice. The tumor growth was observed and the mice were sacrificed 10 weeks later. Tumor tissue was examined by pathology and electron microscopy. AFP was detected in peripheral blood of nude mice. Ten untreated male BALB / c nude mice were randomly selected as control. Immunohistochemistry was used to detect the microvessel density (MVD), normal non-tumor tissue as a control. Results: The AFP of the nude mice was (528.35 ± 12.48) ng / ml, and that of the control group was (135.37 ± 9.27) ng / ml. There was a significant difference between the two groups (P <0.05) The average number of microvessels in each medium magnification field was (32.45 ± 2.38) nude mice. The control group was (9.16 ± 1.21), there was significant difference between the two groups (P <0.05). The model has similar morphological features of primary liver cancer. Conclusion: The human hepatocellular carcinoma xenograft tumor model was established successfully.