心脏细胞培养物中含有相当数量的非心肌细胞,故应用体外培养的心肌细胞作实验性研究必需辨别培养物中的心肌细胞与非心肌细胞。我们用Wistar乳鼠的心脏细胞培养物,从观察活细胞的生长、搏动情况、HE染色的细胞形态、PA S染色显示糖元及琥珀酸脱氧酶染色等几方面对培养物中的心肌细胞与非心肌细胞进行鉴别。 在倒置相差显微镜下观察培养的心肌细胞,自培养48小时起就开始生长,由圆形长成棱形与锥形,并出现单个心肌细胞的自 Cardiac cell cultures contain a significant amount of non-cardiomyocytes, so the use of in vitro cultured cardiomyocytes for experimental studies must discriminate between cardiomyocytes and non-cardiomyocytes in culture. We used cardiac cell cultures of Wistar suckling mice to compare the effects of glycogen and succinate dehydrogenase staining on the growth of cardiomyocytes and pulsatility in HEK cells, PA S staining, Non-cardiomyocytes were identified. The cultured cardiomyocytes were observed under an inverted phase contrast microscope and began to grow 48 hours after the culture, growing from a circle into a prismatic shape and a conical shape, and the appearance of a single cardiomyocyte from