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为探讨 VLDL受体结合域中 8个重复序列在结合 VLDL中所起的作用 ,利用构建的全长VLDL受体 c DNA和缺失 5个重复序列的该受体 c DNA重组表达载体分别导入 CHO细胞中 .RT- PCR可检测到外源性 VLDL受体基因的表达 .受体与配体结合研究表明 ,转染全长 VLDLR重组体的 CHO细胞结合β- VLDL的能力明显高于转染 VLDLR缺失重组体的 CHO细胞 ,表明人VLDL受体在 CHO细胞中能有效表达 ,而缺失 5个重复序列的 VLDL受体基本失去了结合β-VLDL的能力
To investigate the role of 8 repeats in VLDL receptor binding in binding to VLDL, we constructed the full length VLDL receptor c DNA and introduced this receptor c DNA recombinant expression vector with 5 repeats into CHO cells .Expression of exogenous VLDL receptor gene can be detected by RT-PCR.Receptor-ligand binding studies show that CHO cells transfected with full-length VLDLR recombinant CHO cells are significantly more capable of binding to [beta] -VLDL than transfected VLDLR deletion Recombinant CHO cells indicate that human VLDL receptors are efficiently expressed in CHO cells whereas VLDL receptors lacking 5 repeats essentially lose their ability to bind to β-VLDL