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目的评价巢式PCR方法用于输入性疟疾病例检测的应用价值。方法引用Snounou and Perandin F等人描述的引物,对本实验室所冻存标本进行巢式PCR检测,并与厚薄血膜显微镜镜检结果进行比较。结果两种方法结果差异有统计学意义,似然比χ2=21.801,P=0.010,且在分型上较厚薄血膜显微镜镜检方法容易被操作者接受。结论在当前应对输入性疟疾的特殊时期,采用巢式PCR对输入性疟疾进行实验室检测和分型,不仅可以作为显微镜检查的有效补充,并且能够发挥其灵敏度和特异性高的优势。
Objective To evaluate the value of nested PCR in the detection of imported malaria cases. Methods The primers described by Snounou and Perandin F et al were used to detect nested PCR in the frozen samples of the laboratory and compared with the results of the thick and thin blood film microscopy. Results The difference between the two methods was statistically significant, the likelihood ratio χ2 = 21.801, P = 0.010, and in the classification of thicker thin film microscopic examination method easily accepted by the operator. Conclusion In the special period of current response to imported malaria, nested PCR for laboratory detection and typing of imported malaria not only serves as an effective supplement to microscopic examination, but also has the advantage of high sensitivity and specificity.