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以七叶一枝花种子为试材,分别用浓度为0.025%、0.050%、0.100%、0.150%、0.200%的秋水仙素处理七叶一枝花种子12、24、48、60h,研究不同浓度的秋水仙素及不同处理时间对七叶一枝花多倍体诱导的影响,以期为进一步选育七叶一枝花优良多倍体品种提供材料,为探索秋水仙素诱导七叶一枝花多倍体育种研究提供参考。结果表明:秋水仙处理的七叶一枝花根尖均有明显的膨大现象,其中浓度为0.050%秋水仙素处理12h的根尖膨大率最高,为51.69%;秋水仙素处理的七叶一枝花种子的胚根短且粗、胚轴伸长;经秋水仙素处理种子萌发的组培苗植株较对照植株的根茎明显加粗、节间变短。采用流式细胞仪检测发现,秋水仙素处理样品的组培苗的幼根细胞DNA荧光强度约是对照细胞DNA荧光强度的1.88倍,表明经秋水仙素处理的七叶一枝花种子萌发的组培植株细胞染色体出现了部分加倍。
The seeds of the seven-leaf-one flower were used as the test material to treat the seeds of the seven-leaf-one flower at the concentrations of 0.025%, 0.050%, 0.100%, 0.150% and 0.200% respectively for 12, 24, Colchicine and different treatment time on the polyploid Indica plant flower, in order to further seed selection Prunus elegans polyploid varieties provide material, to explore colchicine-induced polyploid breeding of a flower Research for reference. The results showed that the root tips of seven leaves per plant treated with colchicines were obviously inflated. Among them, the root apical swelling rate of colchicines treated with 0.050% colchicine was the highest at 51.69% The embryo of the seed was short and thick, and the hypocotyls were elongated. The tissue culture seedlings germinated by colchicine were significantly thicker than those of the control plants, and the internodes became shorter. Flow cytometry showed that the DNA fluorescence intensity of young root cells of the cultured cells treated with colchicine was about 1.88 times that of the control cells, indicating that the germination of colchicine-treated seedlings Plant cell chromosomes appeared partially doubled.