论文部分内容阅读
本研究以项目组选育的3个紫花含笑新品种“荷瓣墨紫”、“蝶瓣墨紫”和“梅瓣墨紫”为对象,建立了基于ISSR标记的分子鉴别体系。3个UBC系列ISSR标记的扩增产物UBC815-1 000 bp、UBC836-1 200 bp及UBC840-460 bp,可有效鉴别“荷瓣墨紫”、“梅瓣墨紫”和“蝶瓣墨紫”3个新品种;根据UBC844扩增的谱带,可将3个新品种与紫花含笑其它个体区分开来;根据UBC854-1 100 bp标记,可一次性将紫花含笑从含笑、乐昌含笑、深山含笑和金叶含笑4个近缘种中区别出来。ISSR检测结果还表明,含笑属5个种的物种水平的Nei’s基因多样度(h)为0.312 3,Shannon信息指数(I)为0.471 2,显示物种间具有丰富的遗传多样性。5个种种间的Nei遗传距离在0.169 3~0.778 7之间,相似系数在0.483 6~0.786 9之间。采用UPGMA聚类法以遗传距离进行聚类,结果表明:含笑属5个种中,同种的不同个体均能很好地聚在同一种下,显示在分子水平上支持5个种的传统分类方法;分析5个种的亲缘关系,紫花含笑与含笑的亲缘关系最近,而与乐昌含笑的亲缘关系最远,这与前两物种在表型上更为接近相一致。本研究可为紫花含笑杂交育种亲本的选配、嫁接繁殖砧木的选择和种(或品种)的鉴别等提供重要依据。
In this study, we established the ISSR-labeled molecules based on the three new varieties of “Ligustrum”, “Cymbidium purple”, “Petal purple” and “ Identification system. UBC815-1000 bp, UBC836-1 200 bp and UBC840-460 bp were amplified by 3 UBC series ISSR markers, which can effectively identify the ”petal ink purple“, ”Mei Mei ink purple“ and ” According to the amplified band of UBC844, three new varieties can be distinguished from other individuals of Zixia smile. According to the 100 bp mark of UBC854-1, the purple flower can be smoothed out from the smile , Lechang smile, smiling and gold leaf smile four relatives in the distinction. The results of ISSR also showed that Nei’s gene diversity (h) was 0.312 3 and Shannon’s information index (I) was 0.471 2, indicating rich genetic diversity among species. The Nei genetic distances among the five species ranged from 0.169 3 to 0.778 7 with similarity coefficients ranging from 0.483 6 to 0.786 9. The UPGMA clustering method was used to cluster the genetic distance. The results showed that among the five species of Michelia, different individuals of the same species clustered well under the same species, indicating that the traditional classification of five species supported on the molecular level Methods. The genetic relationships among the five species were analyzed. The relationship between the purple flower and the smile was the closest, but the farther away from Lechang than the other two species, which was closer to the phenotypes of the two species. This study may provide an important basis for the selection of crossbred parents, the choice of grafting breeding stock and the identification of species (or breed).