论文部分内容阅读
目的:探讨卵泡生长分化因子-9(GDF-9)在卵泡发育异常及激素水平变化等方面的作用机制。方法:①利用改良Poretsky方法建立高胰岛素血症动物模型(模型组,n=20):hCG×9d+胰岛素×22d。采用下列方法验证模型:测定空腹血糖、空腹胰岛素(FINS)、睾酮(T)、E2、LH、FSH,并计算胰岛素抵抗指数(HOMA1-IR);测定大鼠体重及卵巢重量;HE染色方法观察卵巢病理变化。②采用免疫组化和RT-PCR法检测模型动物及正常对照组(n=20)卵巢组织GDF-9 mRNA及蛋白的表达。结果:①与对照组比较,模型组FPG、FINS、HOMA1-IR、T升高(P<0.05)。②组间体重无统计学差异,但模型组卵巢重量显著大于对照组(P<0.05)。模型组各发育阶段卵泡明显减少,但囊性扩张的卵泡和闭锁卵泡增多,卵泡膜细胞增生,卵泡间质明显增厚。③模型组卵巢组织GDF-9 mRNA和蛋白的表达均显著低于对照组(P<0.05)。结论:高胰岛素血症减弱卵巢组织GDF-9 mRNA和蛋白的表达强度;表达降低的GDF-9可能与PCOS卵泡发育异常及激素水平变化有关。
Objective: To investigate the mechanism of follicular growth and differentiation factor-9 (GDF-9) in follicular dysplasia and hormone levels. Methods: ① The animal models of hyperinsulinemia were established by modified Poretsky method (model group, n = 20): hCG × 9d + insulin × 22d. The model was validated by the following methods: fasting blood glucose, fasting insulin (FINS), testosterone (T), E2, LH and FSH were measured and the insulin resistance index (HOMA1-IR) was calculated; body weight and ovarian weight were measured; Ovarian pathological changes. ② Immunohistochemistry and RT-PCR were used to detect the expression of GDF-9 mRNA and protein in ovarian tissue of model animals and normal control group (n = 20). Results: ①Compared with the control group, FPG, FINS, HOMA1-IR and T increased in the model group (P <0.05). ② There was no significant difference in weight between groups, but the weight of ovary in model group was significantly higher than that of control group (P <0.05). In the model group, the number of follicles in each stage of development was significantly reduced, but the number of cystic dilated follicles and atresia follicles increased, and the number of follicular cells increased significantly. ③ The expression of GDF-9 mRNA and protein in ovarian tissue in model group were significantly lower than those in control group (P <0.05). CONCLUSION: Hyperinsulinemia attenuates the expression of GDF-9 mRNA and protein in ovarian tissue. Decreased expression of GDF-9 may be related to follicular dysplasia and changes in hormone levels in PCOS.