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目的探讨恶性肿瘤组织的氧化-还原态及其抗氧化能力的改变。方法42例原发性消化道恶性肿瘤(食管癌13例、胃癌14例及结直肠癌15例),以其相对应的癌旁正常粘膜组织为对照,测定其谷胱甘肽氧化还原对(GSH、GSSG)的含量及辅酶Ⅱ氧化还原对(NADPH、NADP+)的含量,并据此计算出GSH/GSSG、NADPH/NADP+比值以及GSH/GSSG氧化还原电位值、NADPH/NADP+氧化还原电位值。结果癌组织中的GSH、NADPH含量明显高于癌旁组织(P<0.01)。GSH/GSSG、NADPH/NADP+的氧化-还原电位轻度还原偏移(P<0.05)。而GSSG含量及NADP+含量则与对照组无明显差异(P>0.05)。结论消化道恶性肿瘤组织的GSH、NADPH含量明显升高,提示其抗氧化能力明显增强;而在此基础上,其氧化-还原电位仅较癌旁组织轻度偏向还原方向,提示癌组织内仍存有较明显的氧化应激态。
Objective To investigate the changes of the oxidation-reduction state and antioxidant capacity of malignant tumor tissues. Methods Forty-two patients with primary gastrointestinal malignancies (esophageal cancer in 13, gastric cancer in 14, and colorectal cancer in 15) were enrolled in this study. Their corresponding normal mucosa tissues were used as controls, and their glutathione redox ( GSH and GSSG), and NADPH and NADP +, and calculated GSH / GSSG, NADPH / NADP + ratio, GSH / GSSG redox potential and NADPH / NADP + redox potential. Results The contents of GSH and NADPH in cancer tissues were significantly higher than those in adjacent tissues (P <0.01). GSH / GSSG, NADPH / NADP + oxidation-reduction potential mild reduction bias (P <0.05). However, GSSG content and NADP + content had no significant difference with the control group (P> 0.05). Conclusions The content of GSH and NADPH in the digestive tract malignant tumor was significantly increased, suggesting that its anti-oxidant capacity was significantly enhanced. On the basis of this, the oxidation-reduction potential of the digestive tract malignant tissue was only slightly reduced compared with that of the paracancerous tissue, There is a more obvious oxidative stress.