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目的研究一种多通道、高通量DNA合成控制系统,可实现单次96通道寡核苷酸长链的全自动合成。方法该控制系统以松下FP2系列PLC及其外围功能模块为核心,完成合成文件和序列文件的解析执行,以及自动和手动合成操作的过程控制;基于组态软件和VC6.0开发上位机人机交互界面,完成合成过程配置,以及流量校准、位置校准等参数设置,并与主控系统完成数据交互。结果经15万条DNA应用合成及测序,结果表明合成时间达到4 min/cycle,耦合效率为99%,且单体试剂消耗量减少>50%,合成过程配置灵活。结论 DNA合成仪控制系统可同时实现96个独立通道的寡核苷酸长链的合成,为多通道高通量DNA合成提供了一种高效、灵活、可靠的控制方法。
Objective To study a multi-channel, high-throughput DNA synthesis control system that can fully synthesize a single long-chain oligonucleotide with 96 channels. Method The control system to Panasonic FP2 series PLC and its peripheral function modules as the core, the completion of the synthesis of documents and sequence analysis of the implementation of the document, as well as automatic and manual synthesis of the operation of the process control; based on the configuration software and VC6.0 host computer Interactive interface to complete the synthesis process configuration, as well as traffic calibration, location calibration and other parameters set, and the main control system to complete the data exchange. The results were synthesized and sequenced using 150,000 DNA applications. The results showed that the synthesis time was 4 min / cycle, the coupling efficiency was 99%, and the consumption of monomer reagents was reduced by> 50%. The synthesis process was flexible. Conclusion The DNA synthesizer control system can simultaneously synthesize 96 independent oligonucleotides and provide an efficient, flexible and reliable control method for multichannel high-throughput DNA synthesis.