【摘 要】
：
对采自云南省元谋县的花椰菜丛枝病植原体感病植株进行组织培养。将带菌组培苗分别置于冰箱（4～8 ℃）低温保藏和光照培养箱中常温保藏,经定期观察和继代培养,结果表明：以感病植株
【机 构】
：
云南农业大学植物病理重点实验室,玉溪师范学院资源环境学院,云南省农业科学院热区生态农业研究所
论文部分内容阅读
对采自云南省元谋县的花椰菜丛枝病植原体感病植株进行组织培养。将带菌组培苗分别置于冰箱（4～8 ℃）低温保藏和光照培养箱中常温保藏,经定期观察和继代培养,结果表明：以感病植株的茎段作为组培材料,经丛生芽诱导培养可得到长势较好的花椰菜潜带植原体组培苗;同时,采用低温保藏可延长继代培养的间隔时间（每2～3月进行1次）,期间每月在培养箱中补光3～7 d,在12个月后经PCR检测表明组培苗中仍含有植原体,达到植原体株系长期保存的目的。
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