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目的 :在考察淫羊藿苷 (icariin)于人工胃液中稳定性的基础上 ,研究了肠道内细菌对淫羊藿苷的代谢作用。方法 :于人工胃液或肠内菌培养液中 ,加入淫羊藿苷温孵培养一定时间后 ,以薄板层析、高效液相色谱仪和电喷雾质谱仪 ,对培养物成分做定性分析检查。结果 :淫羊藿苷在人工胃液中有较高的稳定性。离体培养人肠道内细菌可代谢淫羊藿苷 ,且其主要代谢产物为淫羊藿苷的苷元 (icaritin)及其苷元的异戊烯基位置异构体。在大鼠整体实验中 ,从粪便和尿液中均检出一主要代谢产物 ,并初步确定此代谢产物为宝藿苷 (baoliuoside )。结论 :在离体条件下 ,淫羊藿苷可被人肠内菌代谢 ,主要代谢产物为淫羊藿苷的苷元。大鼠灌服淫羊藿苷后 ,吸收入血的主要代谢物为宝藿苷 。
Objective : To study the effect of icariin on the stability of artificial gastric juice, and to study the metabolism of icariin by bacteria in the intestine. METHODS: In artificial gastric juice or intestinal culture medium, icariin was incubated and incubated for a certain period of time. The components of the culture were qualitatively analyzed by thin-layer chromatography, high performance liquid chromatography, and electrospray mass spectrometry. Results : Icariin has high stability in artificial gastric juice. In vitro culture of human intestinal bacteria can metabolize icariin, and its main metabolites are icariin aglycones (icaritin) and isopentenyl isomers isomers. In the whole rat experiment, a major metabolite was detected from feces and urine, and the metabolite was preliminarily identified as baoligoside. Conclusion: In vitro, icariin can be metabolized by human intestinal bacteria, and the main metabolite is aglycone of icariin. After oral administration of icariin to rats, the main metabolite absorbed into the blood was baicalin.