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目的利用Mimox软件分析幽门螺杆菌Lpp20蛋白模拟表位的性质。方法将源于噬菌体随机肽库筛选技术获得的6个Lpp20模拟表位输入Mimox网站,参数设为默认值,进行模拟表位的比对,寻找其共同序列并分析其性质。结果从ClustalW界面比对结果看,氨基酸D、A、S、G、L在模拟表位中出现的频率较高。通过统计学的方法推导出的共同序列为-[-W][PST][LDE]H[SDE][DM]ASG-[LT][YFW][-R]-,第2位置氨基酸W(50%),第7位置氨基酸D(67%),第8位置氨基酸A(50%),第9位置氨基酸S(50%),第12位置氨基酸L(50%)的出现频率较高。通过JalView界面推导出的共同序列为-WPLHSDASG-LYR-,保守性上第6位置S(分值6)、第7位置D(分值5)、第12位置L(分值5)比较高;特异性上第6位置S(分值2.067 059)、第12位置L(分值2.529 612)、第10位置G(分值1.805 491 4)、第3位置P(分值1.761 471 6)比较高;共同性上第7位置D(66%)、第8位置A(50%)、第9位置S(50%)、第10位置G(66%)比较高。结论结合噬菌体筛选技术和生物信息学Mimox工具对抗原模拟表位进行分析并获得较全面的表位信息,为进一步确定抗原表位及研制新型表位疫苗奠定基础。
Objective To analyze the properties of the Helicobacter pylori Lpp20 protein mimotope using Mimox software. Methods Six Lpp20 mimotopes derived from phage random peptide library screening technology were imported into Mimox website. The parameters were set as default values, and the mimotopes were aligned to find their common sequences and analyzed their properties. Results From the results of ClustalW interface comparison, amino acids D, A, S, G and L appeared higher in mimotopes. The common sequence deduced by the statistical method is - [- W] [PST] [LDE] H [SDE] [DM] ASG- [LT] [YFW] [- R] The amino acid D (67%) in the 7th position, the amino acid A in the 8th position (50%), the amino acid S in the 9th position (50%) and the amino acid L (50%) in the 12th position appeared more frequently. The common sequence deduced from the JalView interface is -WPLHSDASG-LYR-. Conservative upper 6th position S (score 6), 7th position D (score 5), 12th position L (score 5) are higher; Specificity was 6th position S (score 2.067 059), 12th position L (score 2.529 612), 10th position G (score 1.805 491 4), 3rd position P (score 1.761 471 6) ; Common position on the seventh D (66%), the eighth position A (50%), the ninth position S (50%), the tenth position G (66%) is relatively high. Conclusion The phage display technique and bioinformatics Mimox tool were used to analyze the antigenic mimotopes and obtain more comprehensive epitope information, which laid the foundation for the further determination of epitopes and the development of new epitope vaccines.