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将淋巴囊肿病病毒(Lymphocystis disease virus,LCDV)主要衣壳蛋白(Majoy capsid protein,MCP)0.6kb基因片段克隆入真核表达载体pEGFP-N_2,得到阳性重组质粒pEGFP-N_2-LCDV0.6,用脂质体法将其转染入真核细胞CHO,并进行瞬时表达,荧光显微镜观察及特异性RT-PCR检测结果表明:已成功将目的片断LCDV MCP 0.6kb转染到CHO细胞,并得到了初步表达。此研究为LCDV基因工程疫苗的研制提供了实验资料。
The 0.6kb gene fragment of major capsid protein (MCP) from Lymphocystis disease virus (LCDV) was cloned into the eukaryotic expression vector pEGFP-N_2 to obtain the recombinant plasmid pEGFP-N_2-LCDV0.6 The liposomes were transfected into eukaryotic CHO cells and transiently expressed. Fluorescence microscopy and specific RT-PCR results showed that 0.6kb of the target fragment was successfully transfected into CHO cells, Preliminary expression. This research provided experimental data for the development of the genetically engineered vaccine of LCDV.