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理想的疫苗应该能刺激在防御病毒感染中起关键作用的T细胞应答。本文作者运用固相法合成1型人类免疫缺陷症病毒(HIV-1)gpl20全部氨基酸序列的40种部分重叠肽,分别与卵白蛋白(OVA)共价连接,并用完全或不完全弗氏佐剂乳化后,肌肉注射40只3～5岁猕猴。共注射2或3针,间隔时间为3周,剂量为100μg,第2针和/或第3针免疫后1～2周采血,周密度梯度离心法分离外周血单个核细胞(PBMC),并作淋巴细胞增殖试验;通过检测PBMC培养上清中白细胞介素2(IL-2)的活性和PBMC中~3H胸苷渗入的放射自显影活性,用算术平均刺激指数(SI)作为评价指标来鉴定HIV-1gp120氨基酸序列中的T细胞激活位点,SI≥4.0被认为是强的T细胞激活表位。另外,在末次
The ideal vaccine should be able to stimulate a T-cell response that plays a key role in the defense against viral infections. The authors used the solid-phase method to synthesize 40 partially overlapping peptides of the entire amino acid sequence of gpl20 of human immunodeficiency virus type 1 (HIV-1), respectively, covalently linked to ovalbumin (OVA) and completely or incomplete Freund’s adjuvant After emulsification, intramuscular injection of 40 3 to 5-year-old macaque. Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation at 1 or 2 weeks after the second and / or the third needle immunization with a total of 2 or 3 needling injections at an interval of 3 weeks at a dose of 100 μg. For lymphocyte proliferation test; By detecting the activity of interleukin 2 (IL-2) in PBMC culture supernatant and the autoradiographic activity of ~ 3H thymidine infiltration in PBMC, using arithmetic average stimulus index (SI) as the evaluation index Identification of T cell activation sites in the amino acid sequence of HIV-1 gp120 with SI> 4.0 is considered a strong T cell activation epitope. In addition, the last time