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目的了解广东省广州市鼠及蝙蝠狂犬病毒的携带情况,分析鼠、蝙蝠作为人类狂犬病潜在传染源的可能性。方法于2013—2015年在广州市公园、医院以及农贸市场捕获褐家鼠及蝙蝠,无菌采集其脑组织样本,用逆转录聚合酶链反应(RT-PCR)和细胞培养分离法检测狂犬病毒。结果本次调查共捕获159只褐家鼠、66只蝙蝠(57只犬蝠、9只伏翼蝠),共获225份脑组织标本,RT-PCR和细胞培养分离法未在采集的脑组织样本中检测出狂犬病毒。结论广州市褐家鼠、犬蝠及伏翼蝠作为狂犬病传染源的可能性较小。
Objective To understand the carriage of rabies and bats in Guangzhou in Guangdong Province and to analyze the possibility of rats and bats being potential sources of human rabies infection. Methods Rattus norvegicus and bats were harvested in Guangzhou Parks, Hospitals and Farmers’ Market from 2013 to 2015. Samples of brain tissue were collected aseptically. Rabies virus was detected by reverse transcription-polymerase chain reaction (RT-PCR) and cell culture isolation . Results A total of 225 brain tissue samples were obtained from 159 Rattus norvegicus, 66 bats (57 canine bats and 9 volt bats) in this survey. RT-PCR and cell culture isolation were not performed on the collected brain tissue Rabies virus was detected in the sample. Conclusions Guangzhou Rattus norvegicus, Canis bats and Vole Fowls are less likely to be source of rabies infection.