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类Tubby蛋白质(Tubby-like protein,TLP)在动植物中广泛存在,暗示其在生命过程中发挥重要的作用。水稻(Oryza sativa)基因组中有14个TLP家族成员,首先制备了这些蛋白质的抗体,用免疫印迹方法检测了它们在水稻叶片不同生长时期的表达情况,揭示其表达模式;然后对Xa21介导的水稻白叶枯病抗性反应不同时间点进行检测,发现OsTLP2、OsTLP7、OsTLP8和OsTLP9等4个蛋白质的表达发生了变化;进一步比较它们在抗病、感病反应和对照处理中的表达情况,发现不同反应间的表达也有区别。该研究结果为阐释水稻TLP在叶片生长过程中的功能,尤其是在水稻-白叶枯病菌互作过程中的作用提供了重要线索。
Tubby-like protein (Tubby-like protein, TLP) widespread in plants and animals, suggesting that it plays an important role in life. Fourteen members of the TLP family in the genome of Oryza sativa were first prepared and antibodies to these proteins were prepared. Their expression patterns at different growth stages of rice leaves were detected by immunoblotting, and their expression patterns were revealed. Then Xa21-mediated The resistance of rice bacterial blight was detected at different time points and the expression of four proteins, OsTLP2, OsTLP7, OsTLP8 and OsTLP9, was detected. The expression of OsTLP2, OsTLP7, OsTLP8 and OsTLP9 were also compared. There was also a difference in the expression of different reactions. The results of this study provide important clues for elucidating the function of TLP in leaf growth, especially in the interaction between rice-leaf blight bacteria.