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目的:建立六味地黄浓缩丸的HPLC指纹图谱。方法:Dikma Diamonsil C18色谱柱(4.6 mm×250 mm,5μm);以乙腈(含0.05%磷酸)-水(含0.05%磷酸)为流动相梯度洗脱;柱温40℃;流速1.0 mL·min-1;检测波长276 nm(0～10min),236 nm(10～40 min),276 nm(40～60 min);进样量20μL。采用Q-TOF-MS-IDA-MS/MS进行色谱峰指认。结果:该方法精密度、稳定性、重复性良好。采用Q-TOF-MS-IDA-MS/MS对22个共有峰中的18个色谱峰进行了定性鉴别。采用该方法测定了10批六味地黄浓缩丸,其相似度均在0.96以上。结论:该研究为六味地黄浓缩丸的全面质量评价奠定了基础。
Objective: To establish the HPLC fingerprints of Liuwei Rehmannia glutinosa pellets. Methods: Dikma Diamonsil C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile (0.05% phosphoric acid) - water The detection wavelength was 276 nm (0-10 min), 236 nm (10-40 min), 276 nm (40-60 min), and the injection volume was 20 μL. Chromatographic peak identification using Q-TOF-MS-IDA-MS / MS. Results: The method has good precision, stability and repeatability. The 18 peaks of 22 common peaks were qualitatively identified by Q-TOF-MS-IDA-MS / MS. The method was used to determine the ten batches of Liu Wei Di Huang concentrated pills, the similarity is above 0.96. Conclusion: This study lays the foundation for the overall quality evaluation of Liuwei Dihuang Pills.