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目的 获取人幽门螺杆菌热休克蛋白60基因（Hsp60），并将它克隆到质粒 pET－22b（+）中进行核苷酸序列分析。方法 利用 PCR技术扩增 Hsp60，并将其定向插入 pET－22b（＋）载体中，通过 DNA序列分析仪进行核苷酸分析。结果DNA序列分析表明，所克隆的Hsp60基因序列与GeneBank公布的一致。结论 获得了序列正确的Hsp60基因，为其重组表达及相关研究奠定了基础。
Objective To obtain the human Helicobacter pylori heat shock protein 60 gene (Hsp60) and cloned into plasmid pET-22b (+) for nucleotide sequence analysis. Methods Hsp60 was amplified by PCR and inserted into pET-22b (+) vector. Nucleotide analysis was performed by DNA sequence analyzer. Results DNA sequence analysis showed that the cloned Hsp60 gene sequence was identical with that published by GeneBank. Conclusion The Hsp60 gene with the correct sequence was obtained, which laid the foundation for its recombinant expression and related research.