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目的探讨线粒体分裂相关蛋白(Drp-1)与糖尿病周围神经病变(DPN)的关系及木丹颗粒的干预作用。方法 50只大鼠随机分为糖尿病对照(DM)组、木丹颗粒(MD)组、α-硫辛酸(LA)组和木丹颗粒联合α-硫辛酸(CT)组,另设正常(NC)组。12周后检测各组坐骨神经传导速度(sNCV)、血清中总超氧化物歧化酶(T-SOD)、谷胱甘肽(GSH)、丙二醛(MDA)和过氧化氢酶(CAT)的水平及坐骨神经中Drp-1、Bax、Bcl-2mRNA相对表达量。结果 NC、DM、MD、LA、CT组Drp-1 mRNA相对表达量分别为(0.99±0.07)、(1.78±0.08)、(1.48±0.09)、(1.44±0.10)和(1.30±0.05);Bax mRNA相对表达量分别为(1.09±0.21)、(7.08±0.86)、(4.95±0.83)、(4.52±0.88)和(2.86±0.54);Bcl-2 mRNA相对表达量分别为(7.80±0.40)、(2.99±0.64)、(4.06±0.81)、(4.09±0.80)和(5.62±0.83)。与NC组比较,DM组T-SOD、GSH、CAT和sNCV降低,MDA升高(P<0.05);与DM组比较,MD、LA及CT组TSOD、GSH、CAT和sNCV升高,MDA降低(P<0.05),其中CT组变化更明显。结论 Drp-1表达升高可能参与DPN发病,木丹颗粒能干预Drp-1表达,减少细胞凋亡,并降低氧化应激水平,对糖尿病大鼠坐骨神经起到一定的保护作用。
Objective To investigate the relationship between mitochondrion-associated protein (Drp-1) and diabetic peripheral neuropathy (DPN) and the intervention of Mudan granules. Methods Fifty rats were randomly divided into diabetic group (DM), MD group, α-lipoic acid (LA) group and KMD group with α-lipoic acid (CT) )group. After 12 weeks, the sciatic nerve conduction velocity (sNCV), total superoxide dismutase (T-SOD), glutathione (GSH), malondialdehyde (MDA) and catalase (CAT) The relative expression of Drp-1, Bax, Bcl-2mRNA in the sciatic nerve and the sciatic nerve were measured. Results The relative expressions of Drp-1 mRNA in NC, DM, MD, LA and CT groups were (0.99 ± 0.07), (1.78 ± 0.08), (1.48 ± 0.09), (1.44 ± 0.10) and The relative expression levels of Bcl-2 mRNA were (1.09 ± 0.21), (7.08 ± 0.86), (4.95 ± 0.83), (4.52 ± 0.88) and (2.86 ± 0.54) ), (2.99 ± 0.64), (4.06 ± 0.81), (4.09 ± 0.80) and (5.62 ± 0.83), respectively. Compared with NC group, the levels of T-SOD, GSH, CAT and sNCV in DM group decreased and MDA increased (P <0.05); Compared with DM group, the levels of TSOD, GSH, CAT and sNCV in MD, (P <0.05), especially in CT group. Conclusion Drp-1 expression may be involved in the pathogenesis of DPN. Mudan granules can interfere with the expression of Drp-1, reduce apoptosis and decrease oxidative stress, which may play a protective role on sciatic nerve in diabetic rats.