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目的:探讨端粒酶(hTERC)和人乳头病毒(HPV)16/18在宫颈癌中的表达及其临床诊断意义。方法:随机选择2009年3月～2012年3月,我院妇产科收治的宫颈癌确诊病例40例作为观察组。同时,随机选择在本院门诊就诊的宫颈诊断正常40例病理标本作为对照组。分别采用聚合酶链反应(PCR)技术和重复片段扩增方法银染定性法(TRAP-PCR),对所有样本宫颈组织中hTERC和HPVl6/18的感染和表达情况进行检测。利用SPSS17.0软件运用Logistic分析各组HPVl6/18和hTERC的感染和表达与宫颈病变的关联性。结果:宫颈癌组hTERC阳性率为60.0%,显著高于正常宫颈组的2.5%(P<0.01);HPV-16/18阳性率达到92.50%,显著高于正常宫颈组阳性率7.50%(P<0.01);宫颈癌易感性与hTERC、HPV16和HPV18阳性率关联关联系数分别为3.94、4.022和4.73,均达到显著水平(P<0.05)。结论:hTERC基因表达和HPV16/18感染与宫颈癌宫颈组织病变有密切的关联性,在宫颈癌发生发展中起重要作用,两者可以作为宫颈癌早期诊断的临床指标之一。
Objective: To investigate the expression and clinical significance of telomerase (hTERC) and human papillomavirus (HPV) 16/18 in cervical cancer. Methods: From March 2009 to March 2012, 40 cases of cervical cancer diagnosed in our department were selected as the observation group. At the same time, randomly selected 40 cases of normal pathological specimens from the clinic of our hospital as the control group. The infection and expression of hTERC and HPV16 / 18 in all specimens were detected by polymerase chain reaction (PCR) and repeated fragment amplification (TRAP-PCR). Logistic analysis was used to analyze the association between HPV16 / 18 and hTERC infection and cervical lesions with SPSS17.0 software. Results: The positive rate of hTERC in cervical cancer was 60.0%, which was significantly higher than that in normal cervix (P <0.01). The positive rate of HPV-16/18 was 92.50%, which was significantly higher than that in normal cervix <0.01). The correlation coefficients of cervical cancer susceptibility and the positive rates of hTERC, HPV16 and HPV18 were 3.94, 4.022 and 4.73, respectively, reaching significant levels (P <0.05). Conclusion: The expression of hTERC gene and HPV16 / 18 infection are closely related to the cervical lesions of cervical cancer, which play an important role in the development of cervical cancer. Both of them may serve as one of the clinical indicators of early diagnosis of cervical cancer.