论文部分内容阅读
目的:探讨单次口服麦冬多糖MDG-1后排泄量的变化。方法:采用异硫氰酸荧光素(FITC)对麦冬多糖MDG-1进行标记(F-MDG-1),测定取代度。采用高效凝胶色谱法(HPGPC)对粪便及尿液内MDG-1含量进行测定。SD-雄性大鼠,给药组按照300 mg.kg-1给予F-MDG-1,空白组给予纯水,分别在0,4,12,24,48,72 h收集尿液及粪便。测定含量。结果:在单次给予F-MDG-1量300 mg.kg-1后,尿液中含量测定在12 h时达到最大排泄量0.808 7 mg,而后逐渐减少,至72 h最低0.105 3 mg;在粪便测定中在12 h达到最大排泄量为21.332 4 mg,而后逐渐减少,至72 h最低0.506 5 mg。结论:采用FITC对MDG-1进行标记,并采用荧光色谱法对MDG-1在排泄物内的含量变化进行研究是可行的。MDG-1基本不被人体吸收,其主要经粪便排泄。
Objective: To investigate the changes of excretion of single oral Ophiopogon japonicus polysaccharide MDG-1. Methods: Radix Isatidis fluorescein isothiocyanate (FITC) was used to label MDG-1 of Radix Ophiopogonis (F-MDG-1) and the degree of substitution was determined. The contents of MDG-1 in feces and urine were determined by high performance gel permeation chromatography (HPGPC). SD-male rats were treated with F-MDG-1 at 300 mg.kg-1 and pure water at 0, 4, 12, 24, 48 and 72 h respectively. Urine and faeces were collected at 0, 4, Determination of content. Results: After a single dose of 300 mg · kg-1 of F-MDG-1, the maximum urinary excretion reached 0.808 7 mg at 12 h and then decreased gradually to a minimum of 0.105 3 mg at 72 h. The maximum excretion reached 21.332 4 mg at 12 h in the stool test and then decreased gradually to a minimum of 0.506 5 mg at 72 h. CONCLUSION: It is feasible to use FITC to label MDG-1 and to study the content of MDG-1 in excrement by fluorescence chromatography. MDG-1 is basically not absorbed by the body, it is mainly excreted by feces.