论文部分内容阅读
芹菜素(AP)是一种天然的抗氧化剂,具有多种生物活性,但是存在药效低、开发利用低等问题。为了提高芹菜素的开发应用和药效,本研究对芹菜素进行结构修饰,制备了芹菜素席夫碱金属衍生物,并采用紫外(UV),红外(UV),差热-热重分析(DSC-DTG)等波谱分析方法表征化合物。采用水杨酸法和邻苯三酚-NBT法评价芹菜素和其衍生物的体外清除羟基自由基(·OH)和超氧自由基(O2-·)活性。通过表征,合成了两种新的芹菜素席夫碱金属配合物,即[Co(C22H16O4N)2(H2O)2]·8H2O和[Zn(C22H16O4N)2(H2O)2]·4H2O配合物,AP、[Co L2]和[Zn L2]清除·OH自由基的IC50为880.65±46.52μg/m L,517.12±16.36μg/m L,633.62±18.95μg/m L;清除O2-·自由基的IC50为116.30±3.94μg/m L,61.13±0.05μg/m L,48.56±0.32μg/m L,合成的配合物相对芹菜素抗氧化活性增强,有望开发出一种新型抗氧化性食品添加剂,为芹菜素的开发及抗氧化剂的研究提供理论研究基础。
Apigenin (AP) is a natural antioxidants, with a variety of biological activity, but there are low efficacy, low utilization of development and other issues. In order to improve the development and application of apigenin and its pharmacodynamics, this paper studied the structural modification of apigenin and prepared the apigenin derivative of apigenin. UV-vis, UV and DTA DSC-DTG) and other spectral analysis of compounds. The hydroxyl radical (· OH) and superoxide radical (O2-) activity of apigenin and its derivatives in vitro were evaluated by salicylic acid method and pyrogallol-NBT method. Two kinds of new complexes were synthesized by characterization of Schiff base of apigenin, namely [Co (C22H16O4N) 2 (H2O) 2] · 8H2O and [Zn (C22H16O4N) 2 (H2O) 2] , [Co L2], and [Zn L2] · IC50 of OH radical was 880.65 ± 46.52μg / m L, 517.12 ± 16.36μg / m L, 633.62 ± 18.95μg / m L; Was 116.30 ± 3.94μg / m L, 61.13 ± 0.05μg / m L and 48.56 ± 0.32μg / m L, respectively. The antioxidative activity of the synthesized complex relative to apigenin was enhanced. It is expected to develop a new antioxidant food additive The development of apigenin and antioxidants provide theoretical basis for the study.