利用高效液相色谱（ＨＰＬＣ）测定了不同光照处理的大豆（Ｇｌｙｃｉｎｅｍａｘ（Ｌ．）Ｍｅｒｒｉ．）幼苗不同组织的异黄酮类含量。子叶中最高，叶片和根部相对较少。子叶的异黄酮以大豆甙和染料木甙及其丙二酰基结合体为主，且在光照条件下，异黄酮含量随光照时间的增加而显著升高；相反，黑暗中的异黄酮含量随苗龄的增加呈下降趋势；当子叶由黑暗转为光照处理以后，异黄酮含量同样随光照时间的增加而升高。在叶片和根部异黄酮含量和种类也因光照条件的不同而有很大差异。光照条件下，叶片中以染料木甙及其丙二酰结合体和黄酮芦丁为主，且随时间增加呈上升趋势；黑暗中的黄化叶片，则以大豆甙和丙二酰结合体为主，但随时间的变化不明显。在幼苗根部，黑暗条件下几乎检测不出异黄酮的存在；光照条件下，则可检测到５种异黄酮，其中以大豆甙元及其衍生物占主要部分。实验证实了光照对大豆异黄酮的积累有明显的促进作用 The contents of isoflavones in different tissues of soybean (Glycine max (L.) Merri.) Seedlings of different light treatments were determined by high performance liquid chromatography (HPLC). Cotyledons highest, leaves and roots are relatively small. Cotyledon isoflavones to daidzein and genistin and malonyl combination of the main, and in the light conditions, the isoflavones content increased significantly with increasing light time; the contrary, the dark with the content of isoflavones When the cotyledons were changed from dark to light, the content of isoflavones also increased with the increase of illumination time. The content and type of isoflavones in the leaves and roots vary greatly depending on the light conditions. Under illumination, genistein and its malonyl conjugate and flavonoid rutin were the main components in the leaves, and showed an upward trend with the increase of time. The yellowing leaves in the dark, with the combination of daidzin and malonyl as Lord, but the change over time is not obvious. In the seedling roots, almost no detectable isoflavone under dark conditions; Under light conditions, five isoflavones were detected, of which daidzein and its derivatives accounted for the major part. Experiments confirmed that light on the accumulation of soy isoflavones had a significant role in promoting