论文部分内容阅读
目的探索拉坦前列素滴眼液中抑菌剂的合理添加量。方法根据药物成分的特征,选择合适的抑菌剂,依照中国药典2015年版抑菌剂效力检查法,采用试验菌株金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、白色念珠菌、黑曲霉,对合用防腐剂苯扎氯铵和苯氧乙醇按照不同加入量,配置后进行抑菌效果检查。结果 A、B、C组联合应用防腐剂都能达到规定的抑菌效果。0.03%苯扎氯铵和0.015%羟苯乙酯联合应用防腐剂对上述5种试验菌的生长有良好的抑制作用,且为最低有效浓度。结论确定0.04%苯扎氯铵和0.03%羟苯乙酯作为拉坦前列素滴眼液的抑菌剂。
Objective To explore the reasonable amount of antibacterial agent in latanoprost eye drops. Methods According to the characteristics of drug components, select appropriate bacteriostat, according to the Chinese Pharmacopoeia 2015 edition of the bacteriostatic efficacy test method, the test strains Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, black Aspergillus, the combined preservatives benzalkonium chloride and phenoxyethanol according to the amount of different, after the configuration of antibacterial effect check. Results A, B, C combined application of preservatives can achieve the desired antibacterial effect. The combination of 0.03% benzalkonium chloride and 0.015% p-hydroxybenzoate had a good inhibitory effect on the growth of the above five kinds of test bacteria, and was the lowest effective concentration. Conclusions 0.04% Benzalkonium chloride and 0.03% Hydroxytophene were identified as bacteriostats for latanoprost eye drops.