论文部分内容阅读
为了区分移植神经细胞和宿主细胞,便于将来在宿主体内对移植细胞进行在体的电生理记录以及其它方面的研究,通过机械损毁的方法,建立了一种特殊的脑损伤模型。结果发现,通过机械损毁的方法,在大鼠大脑皮层形成形态规则的损伤空洞,其模型稳定,重复性好;在空洞内进行干细胞移植,能够长时间存活,移植神经干细胞绝大部分细胞分化为神经元,只有少量细胞分化为胶质细胞,而且移植细胞与宿主细胞分界明显;对移植细胞进行单细胞电生理记录,记录到神经元放电信号。这些结果表明,通过机械损毁的方法,在大鼠大脑皮层成功建立了一个稳定、精确定位移植细胞与宿主细胞界限的脑损伤模型。
In order to distinguish between transplanted nerve cells and host cells, to facilitate the in vivo electrophysiological recording of the transplanted cells in the host and other aspects in the future, a special model of brain injury is established through the method of mechanical damage. The results showed that, by means of mechanical damage, regular morphologically damaged voids were formed in the cerebral cortex of rats, and the model was stable and reproducible. Stem cells were transplanted in the hollow for a long time and most of the cells of the transplanted neural stem cells were differentiated into Only a small number of cells differentiated into glial cells, and the dendritic cells and host cells demarcated significantly. The single cell electrophysiological recording of the transplanted cells recorded the signal of neuron discharge. These results indicate that a successful brain injury model has been successfully established in the cerebral cortex of rats by means of mechanical damage, which can stably and accurately locate the boundaries between the transplanted cells and the host cells.