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目的观察百草枯(paraquate,PQ)对大鼠肺组织中Smad7和SnoN蛋白表达的影响和DHA的干预作用。方法体重200～220g的SPF级雄性Wistar大鼠40只,随机分为4组:正常对照组、模型组、DHA干预组和DHA对照组,每组10只。DHA干预组和DHA对照组,分别经灌胃给予DHA(500mg/kg.bw),正常对照组和模型组给予等体积玉米油,给予DHA第8日,DHA干预组和模型组一次性经灌胃给予50mg/kg.bw PQ染毒,正常对照组和DHA对照组均给予等体积生理盐水。PQ染毒后,持续给予DHA,于染毒后第35日,处死动物,取出肺组织,制备组织匀浆,测定还原性谷胱甘肽(glutathione,GSH)含量,同时制备肺组织切片,免疫组织化学方法观察肺组织中Smad7及SnoN蛋白表达情况。结果各组肺组织中GSH含量无显著差异(P>0.05);与正常对照组比较,模型组大鼠肺组织中Smad7,SnoN蛋白表达水平显著下降(P<0.01,P<0.01);DHA干预组大鼠肺组织中Smad7,SnoN蛋白表达水平明显高于模型组(P<0.01,P<0.05),与正常对照组无显著差别(P>0.05)。结论 DHA能够逆转PQ诱导肺纤维化负向调节因子Smad7和SnoN的减少,对PQ导致的肺组织损伤具有一定的预防性保护作用。
Objective To observe the effect of paraquat (PQ) on the expression of Smad7 and SnoN in lung tissue of rats and the intervention of DHA. Methods Forty SPF male Wistar rats weighing 200-220 g were randomly divided into 4 groups: normal control group, model group, DHA intervention group and DHA control group, with 10 rats in each group. DHA intervention group and DHA control group were given intragastric DHA (500mg / kg.bw), normal control group and model group were given the same volume of corn oil, given DHA on the 8th, DHA intervention group and model group Stomach given 50mg / kg.bw PQ exposure, normal control group and DHA control group were given equal volume of saline. After the PQ exposure, DHA was continuously administered. On the 35th day after the exposure, the animals were sacrificed and the lung tissue was removed to prepare homogenate for determination of the content of glutathione (GSH). At the same time, lung tissue sections were prepared and immunized Histochemical methods were used to observe the expression of Smad7 and SnoN in lung tissue. Results Compared with the normal control group, the expression of Smad7 and SnoN in the lung tissue of the model group was significantly decreased (P <0.01, P <0.01); the level of GSH in the lung tissue of each group was not significantly different (P> 0.05) The expression of Smad7 and SnoN in the lung tissue of rats in the model group was significantly higher than that in the model group (P <0.01, P <0.05), and no significant difference compared with the normal control group (P> 0.05). Conclusions DHA can reverse PQ-induced reduction of Smad7 and SnoN, a negative preventive factor for lung injury induced by PQ.