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目的:探讨短肽型肠内营养制剂(PBEN)对肠黏膜炎大鼠营养状况的影响。方法:利用甲氨喋呤(MTX)建立大鼠肠黏膜炎模型。60只大鼠随机分为6组,即基础饲料(BF)组,短肽型肠内营养制剂(PBEN)组,整蛋白型肠内营养制剂(IPEN)组,MTX+BF组,MTX+PBEN组和MTX+IPEN组。于第0天和第6天,给予MTX+BF组、MTX+PBEN组和MTX+IPEN组腹腔注射10 mg/kg MTX造成大鼠肠道持续损伤。从第1天开始,BF组和MTX+BF组饲喂BF;PBEN组和MTX+PBEN组饲喂PBEN;IPEN组和MTX+IPEN组饲喂IPEN。每组大鼠每天按159.0 k J(38 kcal)/100 g给予膳食。每天记录各组大鼠的体重和进食量。于第11天处死大鼠,检测血浆中D-乳酸、二胺氧化酶(DAO)和血清总蛋白(TP)、清蛋白(ALB)、前清蛋白(PA)、转铁蛋白(TF)和视黄醇结合蛋白(RBP)水平。结果:大鼠进食BF或PBEN、IPEN后各指标间无显著性差异(P>0.05)。MTX造模导致大鼠肠黏膜严重损伤,MTX+BF组各项指标与BF组比有显著性差异(P<0.05)。MTX+PBEN组大鼠体重下降程度低于MTX+BF组和MTX+IPEN组(P<0.05)。第11天时,MTX+PBEN组大鼠血浆D-乳酸和DAO水平均低于MTX+BF组和MTX+IPEN组,且血清TP、ALB、PA、TF和RBP水平均高于MTX+BF组和MTX+IPEN组(P<0.05)。结论:PBEN可有效地改善肠黏膜炎大鼠的营养状况。
Objective: To investigate the effect of short peptide enteral nutrition (PBEN) on the nutritional status of rats with intestinal mucositis. Methods: The model of intestinal mucositis in rats was established by using methotrexate (MTX). Sixty rats were randomly divided into 6 groups: basal diet (BF) group, short peptide enteral nutrition (PBEN) group, whole protein enteral nutrition (IPEN) group, MTX + BF group, MTX + PBEN Group and MTX + IPEN group. On day 0 and day 6, MTX + BF group, MTX + PBEN group and MTX + IPEN group were given intraperitoneal injection of 10 mg / kg MTX to cause continuous intestinal damage in rats. BF was fed BFB and MTX + BF groups on day 1; PBEN was administered to PBEN and MTX + PBEN groups; IPEN and IPX groups were fed IPEN. Each group of rats was given a daily diet of 159.0 kJ (38 kcal) / 100 g. The body weight and food intake of each group were recorded daily. The rats were sacrificed on the 11th day, and the contents of D-lactate, DAO and TP, ALB, TF and TF Retinol binding protein (RBP) levels. Results: There was no significant difference in each index between BFEN and PBEN after IPEN (P> 0.05). MTX induced serious intestinal mucosa injury in rats, MTX + BF group of indicators and BF group had significant difference (P <0.05). The body weight loss of MTX + PBEN group was lower than that of MTX + BF group and MTX + IPEN group (P <0.05). On day 11, the levels of D-lactate and DAO in MTX + PBEN group were lower than those in MTX + BF group and MTX + IPEN group, and the levels of TP, ALB, PA, TF and RBP were higher in MTX + PBEN group than in MTX + BF group MTX + IPEN group (P <0.05). Conclusion: PBEN can effectively improve the nutritional status of rats with intestinal mucositis.