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作者观察了用凝胶过滤技术分离蚯蚓提取物获得的第2、第3和第4组分对MGc803胃癌细胞~3H-TdR参入(单位为cpm)的影响.结果:①第4组分可非常显著地抑制该胃癌细胞的~3H-TdR参入(第4组分2705±488cpm,对照5387±728cpm,P<0.01);②该组分在56℃加热0.5h,上述抑制作用基本消失(加热第4组分5085±490cpm,P>0.05);③第2和第3组分无论加热与否,对该胃癌细胞的~3H-TdR参入均无抑制作用(第2组分5322±292cpm,P>0.005;加热第2组分5341±499cpm,P>0.05;第3组分5036±274cpm,P>0.05;加热第3组分5578±814cpm,P>0.05).实验结果表明,第4组分具有直接抑制MGc803胃癌细胞生长的作用,但该组分缺乏耐热性.
The authors observed the effects of the second, third, and fourth fractions obtained from gel filtration technology on the 3H-TdR incorporation of MGc803 gastric cancer cells (in units of cpm). Results: 1 The 4th component can be very The 3H-TdR incorporation of the gastric cancer cells was significantly inhibited (4705, 488 cpm, 5387±728 cpm, P<0.01). 2 The above-mentioned components disappeared when heated at 56° C. for 0.5 h (heating 4 fractions were 5085±490 cpm, P>0.05); 3 The 2nd and 3rd fractions had no inhibitory effect on ~3H-TdR incorporation into gastric cancer cells with or without heating (the second component was 5322±292 cpm, P> 0.005; heating the second component 5341±499 cpm, P>0.05; the third component 5036±274 cpm, P>0.05; heating the third component 5578±814 cpm, P>0.05). Experimental results show that the fourth component has Directly inhibits the growth of MGc803 gastric cancer cells, but this component lacks heat resistance.