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目的建立一种快速测定疏血通注射液浸提过程中总固体含量的近红外光谱法。方法以水蛭和地龙2种药材浸提过程为例,采用偏最小二乘法(PLS)建立总固体含量的近红外光谱分析校正模型,实现对总固体含量的快速测定。结果近红外光谱在一阶导数结合Karl Norris平滑滤波处理下,建模效果最佳。水蛭总固体含量校正集相关系数(R)为0.810 8,校正集和验证集预测误差均方根(RMSEC、RMSEP)分别为0.583和0.495,交叉验证误差均方根(RMSECV)为0.81,校正集和验证集相对偏差(RSEC、RSEP)分别为6.11%和5.25%;地龙总固体含量校正集R值为0.975 5,RMSEC和RMSEP分别为1.10和1.85,RMSECV为1.61,RSEC和RSEP分别为4.68%和7.80%。结论近红外光谱可用于快速测定疏血通注射液浸提过程中总固体含量,有望推广应用于中药浸提过程的在线质量控制。
Objective To establish a rapid determination of the total solid content of Shuxuetong injection leaching by near infrared spectroscopy. Methods Taking the leaching of two medicinal materials, leeches and earthworms, as an example, partial least square method (PLS) was used to establish the calibration model of total solid content by near infrared spectroscopy to realize the rapid determination of total solid content. Results Near infrared spectroscopy in the first derivative combined with Karl Norris smoothing filter processing, modeling the best. The correlation coefficient (R) of calibration set for total leeches was 0.810 8, RMSEC (RMSEC) of the calibration set and validation set were 0.583 and 0.495, respectively, and RMSECV was 0.81. The calibration set (RSEC, RSEP) of validation set were 6.11% and 5.25%, respectively. The R set value of total solids content was 0.975 5, RMSEC and RMSEP were 1.10 and 1.85 respectively, RMSECV was 1.61, RSEC and RSEP were 4.68 % And 7.80%. Conclusion Near-infrared spectroscopy can be used to determine the total solid content of Shuxuetong injection rapidly, which is expected to be widely applied to the on-line quality control of traditional Chinese medicine extraction.