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16种化学杀雄剂在油菜(B.napus)上的试验表明,0.4%的GA_2单核期处理,1%或1.5%MC_3单核期处理,1.5%KMS-1单核期处理,0.5%乙二胺四乙酸钠单核期处理,能诱导80%以上的不育株。经化学杀雄剂处理后,油莱小孢子发育出现一系列异常现象,此外花药过氧化物同工酶带型少而弱,脱氢酶活力弱等。化学杀雄单低强优势组合湘油11号×466比常规推广品种增产19.2%以上。
The experiments of 16 chemical killing agents on B.napus showed that the treatment with 0.4% of GA 2 mononuclear, 1% or 1.5% MC 3 mononuclear, 1.5% KMS-1 mononuclear and 0.5% Ethylenediaminetetraacetic acid mononuclear treatment, can induce more than 80% of sterile plants. After the chemical killing agent treatment, oillens microspore development showed a series of anomalies, in addition to the anther peroxidase with less and weaker bands, dehydrogenase activity is weak and so on. Chemical kill a single low-strength combination of male superior Xiang You 11 × 466 than conventional varieties to increase 19.2%.