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水温18.2~21.9℃,盐度30~32,在室内200 L塑料水槽中添加不同的碳源(葡萄糖、蔗糖、玉米淀粉、地瓜粉等)形成生物絮团,养殖体重(0.9±0.1)g/只的仿刺参(Apostichopus japonicus)幼参2个月,探讨水体中的生物絮团对其体内主要消化酶和免疫酶活性的影响。结果表明,养殖水体中添加淀粉、蔗糖有利于提高幼参体内消化酶(淀粉酶、蛋白酶)的活性。复合碳源组(葡萄糖∶果糖∶蔗糖∶玉米淀粉∶地瓜粉=0∶3∶4∶2∶1),幼参胃蛋白酶活性(10.63 U/mg)显著高于其它组,复合碳源更利于提高幼参胃蛋白酶活性。复合碳源组(葡萄糖∶果糖∶蔗糖∶玉米淀粉∶地瓜粉=1∶2∶4∶1∶2),幼参体壁中ALP活性最高,为2.66 U/mg;而玉米淀粉组中,幼参体液中SOD活性(204.66 U/mg)显著高于其他各试验组(P<0.05)。添加碳源后制得的生物絮团可以提高幼参机体的免疫功能。
Water temperature was 18.2 ~ 21.9 ℃ and salinity was 30 ~ 32. Different kinds of carbon sources (glucose, sucrose, corn starch, sweet potato powder, etc.) were added into 200 L plastic sink to form biological flocs. The body weight was 0.9 ± 0.1 g / Only Apostichopus japonicus was used for two months to investigate the effect of biological floc in water on the activities of major digestive enzymes and immune enzymes. The results showed that adding starch and sucrose to aquaculture water could improve the activity of digestive enzymes (amylase and protease) in the ginseng. The compound peanut oil (glucose: fructose: sucrose: corn starch: sweet potato powder = 0: 3: 4: 2: 1) had a significantly higher pepsin activity (10.63 U / mg) Improve ginseng pepsin activity. The highest ALP activity was 2.66 U / mg in the body wall of cucumber (P <0.05), while it was 2.66 U / mg in the composite carbon source group (glucose: fructose: sucrose: corn starch: sweet potato powder = 1:2:4:1:2) The SOD activity in reference fluid (204.66 U / mg) was significantly higher than that in other test groups (P <0.05). The biofloc produced after adding carbon source can improve the immune function of the ginseng body.