Objective To investigate whether repeated morphine exposure or prolonged withdrawal could influence operant and spatial leing differentially. Methods Animals were chronically treated with morphine or subjected to morphine withdrawal. Then, they were subjected to two kinds of leing: operant conditioning and spatial leing.Results The acquisition of both simple appetitive and cued operant leing was impaired after repeated morphine treatment. Withdrawal for 5 weeks alleviated the impairments. Single morphine exposure disrupted the retrieval of operant memory but had no effect on rats after 5-week withdrawal. Contrarily, neither chronic morphine exposure nor 5-week withdrawal influenced spatial leing task of the Morris water maze. Nevertheless, the retrieval of spatial memory was impaired by repeated morphine exposure but not by 5-week withdrawal. Conclusion These observations suggest that repeated morphine exposure can influence different types of leing at different aspects, implicating that the formation of opiate addiction may usurp memory mechanisms differentially.