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目的 了解肺癌细胞的HLA抗原表达及肺癌的组织类型和免疫修饰对其的影响。方法 采用免疫组织化学方法和流式细胞术从体内外探讨肺癌组织的HLA DR抗原表达。结果 在 5 6例病理确诊的肺癌患者癌组织中 ,腺癌 76% ( 2 2 /2 9) ,鳞癌 8% ( 2 /2 4)表现为HLA DR抗原阳性 ,而 3例小细胞癌均为阴性。腺癌的阳性率明显高于鳞癌和小细胞癌 (P <0 .0 5 )。HLA DR抗原阳性癌细胞周围可见较多的淋巴细胞浸润 ,二者呈明显的正相关 (P <0 .0 5 )。HLA DR抗原表达与患者的临床分期间未见明显相关。体外实验证明γ 干扰素能明显刺激肺癌细胞株的HLA DR抗原表达 ,并呈明显的浓度依赖。结论 HLA抗原的表达与肺癌的组织分型及局部免疫状况明显相关 ,可能影响宿主对肿瘤的免疫反应
Objective To investigate the expression of HLA antigens in lung cancer cells and the types of lung cancer and the effects of immune modification on them. Methods Immunohistochemistry and flow cytometry were used to investigate HLA DR antigen expression in lung cancer tissues in vitro and in vivo. Results In 56 cases of lung cancer with pathologically confirmed lung cancer, 76% (22/27) of adenocarcinomas and 8% (2 / 2-4) of squamous cell carcinomas showed HLA DR antigen positive whereas 3 cases of small cell carcinoma Negative. The positive rate of adenocarcinoma was significantly higher than that of squamous cell carcinoma and small cell carcinoma (P <0.05). HLA-DR antigen-positive cancer cells around the more visible infiltration of lymphocytes, the two showed a significant positive correlation (P <0. 05). HLA DR antigen expression was not significantly correlated with the patient’s clinical stage. In vitro experiments show that γ interferon can significantly stimulate HLA DR antigen expression in lung cancer cell lines, and showed a significant concentration-dependent. Conclusion The expression of HLA antigen is obviously correlated with the tissue typing and local immune status of lung cancer, which may affect the host immune response to tumor